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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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STEMCELL Technologies Inc anti-cd3 (human cd3+cell depletion kit-rosettesep kit)
Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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STEMCELL Technologies Inc rosettesep ≥95% cd3 − cd56
Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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STEMCELL Technologies Inc antibody complexes bispecific for erythrocyte glycophorin a and leukocyte cd3, cd4, cd19, cd36, and cd66b rosetteseptm
Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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STEMCELL Technologies Inc rosettesep™ cd4
Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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STEMCELL Technologies Inc rosetteseptm dm-l density medium
Summary of NK cell purity and expansion ratio in the NK cell-containing populations.
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Image Search Results


Summary of NK cell purity and expansion ratio in the NK cell-containing populations.

Journal: Regenerative Therapy

Article Title: Establishment of an efficient ex vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors

doi: 10.1016/j.reth.2022.07.001

Figure Lengend Snippet: Summary of NK cell purity and expansion ratio in the NK cell-containing populations.

Article Snippet: The CD3 fraction of the PBMCs was depleted by RosetteSepTM Human CD3 Depletion Cocktail (STEMCELL Technologies, Vancouver, Canada).

Techniques:

Ex vivo expansion of human peripheral blood-derived NK cells by defined cytokine cocktail and NK cell activating receptor stimulation. Human CD3-depleted PBMCs were expanded with serum-free medium containing the defined cytokine cocktail and 10% autologous plasma on antibody immobilization plates. The dot plots depict the flow cytometric analysis data of CD3-and/or CD56-expressing cells. Day 0 indicates the CD3-depleted PBMCs. Day 14 indicates the 14-day expansion in the specific conditions. Ctr, non-immobilized; αCD16, anti-CD16 antibody; αNKp46, anti-NKp46 antibody; αCD16/αNKp46, anti-CD16/NKp46 antibody. Top, middle, and bottom panels indicate Donor 1, 2, and 3, respectively.

Journal: Regenerative Therapy

Article Title: Establishment of an efficient ex vivo expansion strategy for human natural killer cells stimulated by defined cytokine cocktail and antibodies against natural killer cell activating receptors

doi: 10.1016/j.reth.2022.07.001

Figure Lengend Snippet: Ex vivo expansion of human peripheral blood-derived NK cells by defined cytokine cocktail and NK cell activating receptor stimulation. Human CD3-depleted PBMCs were expanded with serum-free medium containing the defined cytokine cocktail and 10% autologous plasma on antibody immobilization plates. The dot plots depict the flow cytometric analysis data of CD3-and/or CD56-expressing cells. Day 0 indicates the CD3-depleted PBMCs. Day 14 indicates the 14-day expansion in the specific conditions. Ctr, non-immobilized; αCD16, anti-CD16 antibody; αNKp46, anti-NKp46 antibody; αCD16/αNKp46, anti-CD16/NKp46 antibody. Top, middle, and bottom panels indicate Donor 1, 2, and 3, respectively.

Article Snippet: The CD3 fraction of the PBMCs was depleted by RosetteSepTM Human CD3 Depletion Cocktail (STEMCELL Technologies, Vancouver, Canada).

Techniques: Ex Vivo, Derivative Assay, Clinical Proteomics, Expressing